Fig. 5: PKA inhibition in SSCs only modestly increases trabecular bone mass.
From: Protein kinase A is a dependent factor and therapeutic target in mouse models of fibrous dysplasia
a Schematic representation of the Tet-PKI/Prrx1-Cre (PKI mice) transgenic mouse model, which is designed to express PKA inhibitor peptide (PKI) in a tissue-specific manner upon Dox administration. b Study regimen outlining the Dox treatment schedule for PKI and control mice, along with the timing of sample collection (V video, H histology, TC tissue collection, SC serum collection). c qPCR analysis of limb bones confirming the transcription of PKI mRNA in PKI mice (Control n = 4 and PKI n = 8, biologically independent samples, unpaired two-tailed t-test). d Immunofluorescence staining of tibias showing EGFP-tag expression, verifying successful PKI expression in PKI mice. Three independent experiments were performed. e Immunoblotting demonstrating the suppression of PKA phosphorylation in limb bone. Three independent experiments were performed. f Representative images of limb morphology and g μCT images of hindlimb showing no significant differences in limb swelling or bone deformity between PKI mice and littermate controls. h H&E staining and i TRAP staining of tibias revealing no significant differences in bone histomorphology or osteoclast activity between PKI mice and littermate controls. j Quantitative analysis of the number of adipocytes in tibias of PKI mice (Control n = 4 and PKI n = 5, biologically independent samples, unpaired two-tailed t-test). k, l Representative three-dimensional reconstructions and quantitative analyses of cortical bone and skull (Control n = 5 and PKI n = 9, biologically independent samples, unpaired two-tailed t-test). m, n Representative three-dimensional reconstructions and quantitative analyses of trabecular bone (Control n = 5 and PKI n = 9, biologically independent samples, unpaired two-tailed t-test). The data are presented as box-and-whisker plots, with boxes representing the interquartile range (25th–75th percentiles), the minimum and maximum values reached by bars, the median plotted as a line in the middle, and the mean marked as “+”. Source data are provided as a Source Data file.
