Table 1 Kinetic parameters for CjPglB and DmPglB with GalNAc5(Glc)GlcNAc LLOs

From: Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria

ssOST

Acceptor sequon

kcat (h−1)

KM (μM)

CjPglB

DQNAT

0.42 ± 0.08

10.7 ± 0.98

DmPglB

DQNAT

0.33 ± 0.05

4.30 ± 0.95

CjPglB

QYNST

n.a.

n.a.

DmPglB

QYNST

0.24 ± 0.02

5.16 ± 1.13

  1. aKM and kcat values calculated from in vitro glycosylation data shown in Supplementary Fig. 9. Reactions were performed using extracted GalNAc5(Glc)GlcNAc LLOs and fluorescent TAMRA-GSDQNATF-NH2 or TAMRA-GQYNSTAF-NH2 as substrates. Data are the average of technical replicates (n = 3) ± SD. In the case of CjPglB with QYNST sequon, no activity (n.a.) was detected.
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