Fig. 1: Characterization of lipid nanoparticles. | Nature Communications

Fig. 1: Characterization of lipid nanoparticles.

From: Targeting modulation of the choroid plexus blood-CSF barrier and CSF hypersecretion via lipid nanoparticle-mediated co-delivery of siRNA and resveratrol

Fig. 1

a Gel retardation assay of various siR@polyplexs at different weight ratios. b Evaluation of the protective effect of polyplexs on siRNA against serum. c TEM images showing the morphic changes of siR@sPEI incubated with or without 10 mM GSH. Scale bar, 100 nm. d Hydration particle size and TEM image of siR/RSV@TNP. Scale bar, 50 nm. e Variations in the particle size of siR/RSV@TNP in H2O, PBS, and 10% serum after 24 h of incubation at 37 °C. f, g Lysosome escape test, scale bar 20 μm. h Cellular uptake of free Cy5-siR, Cy5-siR/RSV@NP, Cy5-siR/RSV@TNP, n = 3 biological replicates for each group. i Ex vivo imaging of AD mice brains excised at 2 h post-injection. j Average radiant efficiency of DiD@NP and DiD@TNP group, n = 3 biological replicates for each group. k Cell viability of siR/RSV@TNP, n = 3 biological replicates for each group. All data are presented as mean ± SD. Two-tailed Student’s t test and One-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test or Dunnett’s multiple comparisons test were used for the statistical comparison between the two groups and among multiple groups, respectively. Statistical analyses were conducted with a 95% confidence interval, and significance was defined as p < 0.05. Source data are provided as a Source Data file.

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