Fig. 5: siR/RSV@TNP inhibited hydrocephalus in vivo. | Nature Communications

Fig. 5: siR/RSV@TNP inhibited hydrocephalus in vivo.

From: Targeting modulation of the choroid plexus blood-CSF barrier and CSF hypersecretion via lipid nanoparticle-mediated co-delivery of siRNA and resveratrol

Fig. 5

a Illustrative T2-weighted images (White arrow) and three-dimensional reconstructions of the lateral ventricles were acquired three days later in Ctrl, hydrocephalus, and hydrocephalus treated with various nanomaterials groups. Scale bar, 250 mm. b Measurement of lateral ventricle volumes using associated T2-weighted images (n = 5 animals per group). c Expression of SPAK in the choroid plexus after treatments, n = 5 biological replicates for each group. d Immunofluorescence SPAK in the choroid plexus. Scale bar, 20 μm. e Immunofluorescence pSPAK in the choroid plexus. Scale bar, 10 μm. f, g Expression of pro-inflammatory cytokines CCL2 and IL-6 in the brain after treatments, n = 3 biological replicates for each group, respectively. All data are presented as mean ± SD. One-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test or Dunnett’s multiple comparisons test were used for the statistical comparison between the two groups and among multiple groups, respectively. Statistical analyses were conducted with a 95% confidence interval, and significance was defined as p < 0.05. Source data are provided as a Source Data file.

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