Fig. 6: Changes in the transcriptome of choroid plexus in hydrocephalus mice treated with siR/RSV@TNP. | Nature Communications

Fig. 6: Changes in the transcriptome of choroid plexus in hydrocephalus mice treated with siR/RSV@TNP.

From: Targeting modulation of the choroid plexus blood-CSF barrier and CSF hypersecretion via lipid nanoparticle-mediated co-delivery of siRNA and resveratrol

Fig. 6

a Heat map of differentially expressed gene (DEGs) in hydrocephalus and siR/RSV@TNP treated group. b Volcano map of DEGs. GO enrichment analysis of the gene functions of downregulated (c) and upregulated (d) DEGs. e Downregulated and f upregulated DEGs enriched in the KEGG pathway. g The upregulated DEGs are involved in tight junctions, n = 3 biological replicates for each group. h The downregulated DEGs are involved in myeloid leukocyte migration and ChP inflammation, n = 3 biological replicates for each group. i Immunofluorescence staining of ZO-1 in the choroid plexus. Scale bar, 20 μm. j Immunofluorescence staining of IBA-1in the choroid plexus. Scale bar, 40 μm. All data are presented as mean ± SD. One-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test or Dunnett’s multiple comparisons test were used for the statistical comparison between the two groups and among multiple groups, respectively. Statistical analyses were conducted with a 95% confidence interval, and significance was defined as p < 0.05. Source data are provided as a Source Data file.

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