Fig. 1: Study overview.

a Representative images of astrocytes stained with the Cell Painting dyes. Scale bar = 50 μm. b Schematic of strategy for capturing NeuroPainting high-dimensional image-based profiles from a large cohort of iPSCs. In batches of 44, iPSCs were differentiated into NPCs, neurons, and astrocytes and then plated in 384-well microplates in a randomized orientation for downstream image acquisition. CellProfiler was used to process images and extract morphological features. We imaged one batch for each cell type.