Fig. 1: P. abramus bat and mink ACE2, but not DPP4, enable HKU5 pseudovirus entry.
From: HKU5 bat merbecoviruses engage bat and mink ACE2 as entry receptors
a Phylogenetic analysis of the full-length amino acid sequences of coronavirus spikes from representative subgenera. Hosts and receptors are indicated. Asterisks (*) highlight bat coronaviruses used in this study. Animal illustrations were sourced from BioRender (https://BioRender.com/d86esp4). b HEK-293T cells were transiently transfected with the indicated animal DPP4 constructs, and infection of each spike pseudovirus was assessed. MERS-CoV pseudovirus uses diverse DPP4 orthologs, but other spike pseudoviruses do not use DPP4. c HEK-293T or BHK-21 cells were transiently transfected with the indicated animal ACE2 constructs. Pseudotyped virus infection with coronavirus spikes revealed that HKU5 uses P. abramus and N. vison ACE2 for entry. In contrast, SARS-CoV-2 uses diverse ACE2 orthologs. The mean of three technical replicates is plotted from one of two independent experiments. RLU relative light units, Sbc sarbecovirus, Merbc merbecovirus, Nobc nobecovirus, Emc embecovirus. Source data are provided as a Source Data file.
