Fig. 3: Cryo-EM structure and molecular dynamics of native PopA confirm lipid-trapping features.
From: A porin-like protein used by bacterial predators defines a wider lipid-trapping superfamily
A Cryo-EM density map of native PopAhis, coloured by local resolution. The gross features match those of x-ray determined refolded PopA; the map (yellow, righthand side) is demonstrative of eleven modelled detergent molecules. B PopA simulated in a model outer membrane (upper), showing moderate deformation of the bilayer (lower). C Comparison of experimental alkyl chains (left), exemplar simulation lipids (centre) and density of lipids averaged over 3 500 ns molecular dynamics simulations (right). D Simulation of PopA with palmitate (PAL) and contact time with residues of pocket. E Liposome swelling assays of PopA (light bars) relative to E. coli OmpF (dark bars), illustrating significant permeability to glycine and alanine only. Liposome swelling assays show an average of three technical replicates.
