Fig. 2: VAMP5 KO doesn’t impair the stemness of ESCs.

a–d Representative western blot images of VAMP5 KO potentially impair the related markers expression in hESCs (a) and also after differentiated into mesoderm (MES) (b), endoderm (END), (c) and ectoderm (ECT) (d). Data are represented as mean ± SEM, n = 3 biological replicates in each group. Student’s two tailed t test was used. No adjustments for multiple comparisons were made, as only two groups were compared. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. e PCA of the transcriptomic comparison of VAMP5 WT and KO in hESCs and followed differentiated three germ layers, n = 3 in each cell type. f Representative heatmap of hESCs and three germ layers related markers comparison of VAMP5 WT and KO in hESCs and followed differentiated three germ layers. g Immunofluorescence staining for lineage differentiation from VAMP5 WT and KO hESCs to mesoderm derived smooth muscles cells (markers of α-SMA and Transgelin), endoderm derived alveolar type II cells (markers of EpCAM and SP-B), and ectoderm derived neuron cells (β3 tubulin and MAP2). The results are representative of three independent experiments. Scale bars, 50 μm. KO, knock-out; WT, wild-type.