Fig. 3: Characterization of the photothermal properties and Fenton reaction of FeS@SRB. | Nature Communications

Fig. 3: Characterization of the photothermal properties and Fenton reaction of FeS@SRB.

From: Living therapeutics of nonpathogenic bacteria as biosynthesis factory and active carriers for enhancing tumor-targeted therapy

Fig. 3

A Effect of FeS@SRB concentration on the photothermal effect (808 nm, 1.5 W cm-2). n = 3 independent samples. Data are presented as mean ± SD. B Temperature profiles of FeS@SRB suspension (OD600 = 0.1) under five consecutive on-off cycles of laser irradiation (808 nm, 1.5 W cm2). C Heating-cooling cycle curve and the corresponding linear fitting curve of the negative natural logarithm of time versus driving force temperature. DF Detection of •OH generation of FeS@SRB (OD600 = 0.1) using MB probe at (D) pH 7.4, (E) pH 5.5 conditions, and (F) corresponding statistical analysis. n = 3 independent samples. Data are presented as mean ± SD. G UV-vis absorption spectra of different groups reacting with TMB (here heart symbol represents FeS@SRB). The inset showed the color in a different system. H Statistical analysis of absorbance in different groups at 652 nm. n = 3 independent samples. Data are presented as mean ± SD. I ESR profiles of generated •OH in different groups. J Cell viability of HUVECs and L929 cells after co-incubation with SRB for 24 h, respectively. n = 3 biological independent replicates. Data are presented as mean ± SD. K Cell viability of 4T1 cells, and (L) B16F10 cells with different treatments. n = 3 biological independent replicates. Data are presented as mean ± SD. Statistical significance was calculated by one-way ANOVA. **P < 0.05; ***P < 0.001; ****P < 0.0001. Source data are provided as a Source Data file.

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