Fig. 4: Transcriptome analysis of 4T1 cells treated with FeS@SRB + NIR. | Nature Communications

Fig. 4: Transcriptome analysis of 4T1 cells treated with FeS@SRB + NIR.

From: Living therapeutics of nonpathogenic bacteria as biosynthesis factory and active carriers for enhancing tumor-targeted therapy

Fig. 4

A Volcano plot of DEGs identified at P < 0.05 between the treatments of PBS and FeS@SRB + NIR by RNA-seq analysis. B A cluster analysis of the related genes in upregulated and downregulated DEGs in the FeS@SRB + NIR group. n = 3 biological independent replicates. C GO term enrichment analysis and (D) KEGG enrichment analysis of different expressed genes in PBS and FeS@SRB + NIR-treated 4T1 cells. n = 3 biological independent replicates, one-sided hypergeometric test. (E−K) The relative mRNA expression of (E) HSP70/90 (Hspa1a/Hsp90aa1), (F) GPX4 (Gpx4), (G) CAT (Cat), (H) P53 (Trp53), (I) NF-κB (Nfkb1), (J) ACSL4 (Acsl4), and (K) GADD45 (Gadd45a) treated with PBS, FeS@SRB, and FeS@SRB + NIR, respectively. G1: PBS, G2: FeS@SRB, G3: FeS@SRB + NIR. n = 3 biological independent replicates. Data are presented as mean ± SD. Source data are provided as a Source Data file.

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