Fig. 6: Tumor chemotactic property of FeS@SRB in vivo using subcutaneous 4T1 tumor-bearing mice model. | Nature Communications

Fig. 6: Tumor chemotactic property of FeS@SRB in vivo using subcutaneous 4T1 tumor-bearing mice model.

From: Living therapeutics of nonpathogenic bacteria as biosynthesis factory and active carriers for enhancing tumor-targeted therapy

Fig. 6

A Representative fluorescence image of subcutaneous 4T1 tumor-bearing mice at different time after intravenous injection with IR780-labeled SRB, FeS@BSA and FeS@SRB, respectively. B Tumor-targeted delivery efficiency of SRB, FeS@BSA and FeS@SRB via i.v. injection based on FI statistics. n = 3 biological independent replicates. Data are presented as mean ± SD. C, D Biodistribution of FeS@SRB at 60 h post-i.v. injection assessed by plate coating method. C Photos of bacteria colonies after diluted tumor and organ homogenates coated on agar plates and incubated at 37 °C for 24 h (diluted: 12 times), and (D) corresponding bacteria colony counts in major organs and tumor tissues. n = 3 biological independent replicates. Data are presented as mean ± SD. E Gram staining assay for determining the bacterial distribution in individual organs and tumor tissue at 48 h post-i.v. injection (the blue cycles indicated bacteria colony). Scale bar: 100 μm. Statistical significance of (B) was calculated by one-way ANOVA. ****P < 0.0001, and ns, not significant. Source data are provided as a Source Data file.

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