Fig. 6: Dihydroxylation of 24-methyldesmosterol (2) by CYP87G1 (W22H) and CYP88C7 (W1H). | Nature Communications

Fig. 6: Dihydroxylation of 24-methyldesmosterol (2) by CYP87G1 (W22H) and CYP88C7 (W1H).

From: Phylogenomics and metabolic engineering reveal a conserved gene cluster in Solanaceae plants for withanolide biosynthesis

Fig. 6

a Proposed formation of intermediates and shunt products in N. benthamiana and S. cerevisiae during the dihydroxylation of 24-methyldesmosterol (2). Dashed arrows indicate alternative pathways to N. benthamiana shunt products. b Relative levels of identified compounds in GC-MS chromatograms of N. benthamiana (green) and S. cerevisiae (yellow) upon co-expression of genes from P. pruinosa (Pp). For each compound, peak areas were normalised to internal standard and sample dry weight (either N. benthamiana leaf dry weight or yeast dry cell weight) and converted to relative amounts by setting the highest mean value of each compound for each host organism to 100%. Bar plots show means ± SEM and data points of three biological replicates. W22H: withanolide biosynthesis 22-hydroxylase; W1H: withanolide biosynthesis 1-hydroxylase. Budding_yeast icon by umasstr (https://github.com/umasstr) is licensed under CC0 (https://creativecommons.org/publicdomain/zero/1.0/) and was used without modifications. Nicotiana_benthamiana icon by Connor-Tansley (https://github.com/Ctansley) is licensed under CC-BY 4.0 Unported (https://creativecommons.org/licenses/by/4.0/) and was used without modifications. Source data are provided as a Source Data file.

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