Table 2 The repair patterns of different sgRNAs
From: Refined DNA repair manipulation enables a universal knock-in strategy in mouse embryos
Gene/sgRNA | NHEJ (%) | MMEJ (%) | WT (%) | NHEJ/MMEJ (N/M) |
|---|---|---|---|---|
Actb-sgRNA3 | 49.76 | 50.24 | 0.00 | 1.00 |
Actb-sgRNA7 | 56.05 | 27.13 | 16.82 | 2.07 |
Actb-sgRNA8 | 55.26 | 33.00 | 11.74 | 1.67 |
Cdx2-sgRNA2 | 37.50 | 52.59 | 9.91 | 0.71 |
Cdx2-sgRNA3 | 63.38 | 26.60 | 46.79 | 2.38 |
Cdx2-sgRNA6 | 18.87 | 81.13 | 0.00 | 0.23 |
Sod1-A4-sgRNA1 | 46.62 | 53.38 | 0.00 | 0.87 |
Sod1-A4-sgRNA3 | 58.07 | 41.93 | 0.00 | 1.39 |
Sod1-A4-sgRNA5 | 17.28 | 82.72 | 0.00 | 0.21 |
Sod1-G93-sgRNA2 | 30.47 | 65.08 | 4.45 | 0.47 |
Sod1-G93-sgRNA3 | 45.69 | 48.31 | 6.00 | 0.95 |
Sod1-G93-sgRNA5 | 38.55 | 14.09 | 47.36 | 2.74 |
H3.3-sgRNA2 | 13.40 | 76.60 | 10.00 | 0.17 |
H3.3-sgRNA4 | 46.82 | 44.00 | 9.18 | 1.07 |
Cdk4-sgRNA1 | 18.25 | 81.75 | 0.00 | 0.22 |
Gata6-sgRNA1 | 35.59 | 64.41 | 0.00 | 0.59 |
Dpap5-sgRNA4 | 38.01 | 41.30 | 20.69 | 0.92 |
H2afz-sgRNA3 | 47.39 | 50.35 | 2.26 | 0.94 |
Actb-sgRNA2 | 51.94 | 41.75 | 6.31 | 1.24 |
Actb-sgRNA6 | 36.50 | 27.95 | 35.55 | 1.31 |
Tubb5-sgRNA2 | 24.06 | 14.39 | 61.55 | 1.67 |
Gata6-sgRNA4 | 48.96 | 25.82 | 25.22 | 1.89 |
Dppa3-sgRNA1 | 60.14 | 24.02 | 15.84 | 2.50 |
Cdk4-sgRNA3 | 75.41 | 24.59 | 0.00 | 3.07 |
Gata6-sgRNA5 | 58.03 | 17.50 | 24.47 | 3.32 |
Cenpe-sgRNA | 85.23 | 12.68 | 2.09 | 6.72 |
Slbp-sgRNA | 49.36 | 29.40 | 21.24 | 1.68 |
