Fig. 3: Biochemical and metabolic properties of RIF-resistant M. smegmatis (FluxRIF) bacilli.
A Targeted metabolomics profiles focusing on intermediates in trehalose metabolism, glycolysis, and the pentose phosphate pathway in naïve M. smegmatis bacilli and ten FluxRIF bacilli. Data points represent mean values ± s.e.m. of 10 biological replicates. B Fold change of treS mRNA expression in naïve M. smegmatis and FluxRIF bacilli under RIF treatment conditions relative to untreated conditions. Data points represent mean values ± s.e.m. of 10 biological replicates. C Membrane potential (ΔΨm) of naïve M. smegmatis and FluxRIF bacilli measured after treatment with 30 µg/mL RIF by flow cytometry. The percentage of bacilli exhibiting high and low ΔΨm for each strain is shown relative to the untreated condition. Data points represent mean values ± s.e.m. of biological triplicates of naïve bacilli and 10 biological replicates of FluxRIF bacilli. D Intrabacterial ATP concentrations in FluxRIF bacilli, expressed as a fold change relative to naïve bacilli. Data points represent mean values ± s.e.m. of biological triplicates of naïve bacilli and 10 biological replicates of FluxRIF bacilli. E Relative RIF permeability and EtBr permeability kinetics of naïve M. smegmatis or FluxRIF bacilli. Data points represent mean values ± s.e.m. of biological triplicates of naïve bacilli and 10 biological replicates of FluxRIF bacilli. p values were determined by Student’s unpaired t test with Welch’s correction, ns, not significant. Source data are provided as a Source Data file.
