Fig. 6: The role of trehalose catalytic shift in the emergence of antibiotic cross-resistance. | Nature Communications

Fig. 6: The role of trehalose catalytic shift in the emergence of antibiotic cross-resistance.

From: Trehalose catalytic shift inherently enhances phenotypic heterogeneity and multidrug resistance in Mycobacterium tuberculosis

Fig. 6

A IC50 values for INH (left panel) and BDQ (right panel) were measured in naïve M. smegmatis, FluxRIF, and ItreSFlux bacilli, both with and without ATc treatment. Data points represent mean values ± s.e.m. of biological triplicates. B Spot assay on m7H10 containing 10X MIC of INH (isoniazid) or BDQ (bedaquiline), using naïve M. smegmatis, FluxRIF, and ItreSFlux bacilli. C IC50 values for BDQ were determined for naïve M. smegmatis and two selected FluxINH bacilli. Data points represent mean values ± s.e.m. of biological triplicates. D Spot assay on m7H10 containing 10X MIC of BDQ (left panel) with naïve M. smegmatis and FluxINH bacilli. Data points represent mean values ± s.e.m. of 6 biological replicates of naïve bacilli and 10 biological replicates of FluxINH bacilli. The right panel displayed the average colony diameters and standard deviations of colonies grown on m7H10 containing 10X MIC of BDQ. Source data are provided as a Source Data File.

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