Fig. 4: GMP facilitates the binding of ATP to GMPK.
From: Comprehensive profiling of the catalytic conformations of human Guanylate kinase
a Raw ITC binding isotherms of ATPγS binding to GMPK (blue curve) and GMPK-GMP mixtures (magenta curve). b Fitted ITC binding isotherms, with Kd values and associated errors presented from triplicate experiments. c1 H-15N HSQC spectra of ATPγS titration against GMPK and GMPK-GMP mixtures, with colors representing molar ratios of ATPγS to GMPK: red (0), cyan (0.5), pink (1), and purple (2). d Raw ITC binding isotherms of ADP binding to GMPK (gray curve) and GMPK-GMP mixtures (pink curve). e Structural superposition of apo, ADP-bound and GMP-ATPγS-bound GMPK. f Structural superposition of apo, GMP-bound and GMP-ATPγS-bound GMPK.
