Fig. 3: Pathogenicity of bovine HPAI A(H5N1) 2.3.4.4b viruses in mice. | Nature Communications

Fig. 3: Pathogenicity of bovine HPAI A(H5N1) 2.3.4.4b viruses in mice.

From: Genotype B3.13 influenza A(H5N1) viruses isolated from dairy cattle demonstrate high virulence in laboratory models, but retain avian virus-like properties

Fig. 3

a, b BALB/c mice (6-8 weeks old) were lightly anesthetized and inoculated IN with 10-fold-serial dilutions of bovine/OH/439 (H5N1) and bovine/TX/98638 (H5N1) viruses to determine MLD50 for each (n = 3/virus dose). c Virus titer in tissues from infected mice was determined by TCID50 assay in MDCK cells shown as the mean ± SD with individual mouse data represented by shapes. Immunohistochemistry of fixed tissues from A(H5N1) 2.3.4.4b virus-infected mice (n = 3/group) showed virus antigen in nasal epithelium (d, scale bar, 50 µm), lung (e, scale bar, 200 µm), brain (f, scale bar, 2 mm), spinal cord (g, scale bar, 1 mm), liver (h, scale bar, 100 µm), and brown adipose tissue (BAT) (i, scale bar, 100 µm).

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