Fig. 6: Antigenic and serologic characterization of bovine HPAI A(H5N1) 2.3.4.4b viruses. | Nature Communications

Fig. 6: Antigenic and serologic characterization of bovine HPAI A(H5N1) 2.3.4.4b viruses.

From: Genotype B3.13 influenza A(H5N1) viruses isolated from dairy cattle demonstrate high virulence in laboratory models, but retain avian virus-like properties

Fig. 6

a Antigenic properties of 16 bovine HPAI A(H5N1) 2.3.4.4b viruses were determined in HI assay with post-infection ferret antisera generated against the WHO-recommended CVVs of clade 2.3.4.4b [Astrakhan/3212 (H5N8), ck/Ghana/21 (H5N1), wigeon/SC/345 (H5N1),], and 2.3.4.4c [gyrfalcon/41088 (H5N8)] and representative avian eagle/FL/W22 (H5N1) virus. The data are shown as the mean ± SD with HI titers for each virus as individual circles. The dotted line indicates limit of detection for the assay (HI titer = 10). (★) indicates the endpoint titer of homologous ferret sera to the indicated CVV. b The presence of cross-reactive HA antibodies against bovine A(H5N1) 2.3.4.4b viruses was examined in a set of human sera obtained from a Phase 1 clade 2.3.4.4c H5 vaccine trial. Individuals (aged 18 to 50 years) were vaccinated with adjuvanted 15 µg of the HA derived from gyrfalcon/41088 (H5N8) 2.3.4.4c antigen (n = 20). The data are shown as the mean ± SD. c A set of human sera (n = 24) was obtained from BioIVT (individuals, aged 18 to 46 years, vaccination history unknown). NA activity inhibiting (NI) antibody levels in human serum samples against N1 NA protein derived from CA/09 (H1N1)pdm09, bovine/OH/439 (H5N1), and eagle/FL/W22 (H5N1) viruses (as measured by ELLA assay). The data are shown as geometric mean titer (line) of the individual IC50 NI titers (dots). Statistical significance was determined by two-way ANOVA with Tukey’s post-test for multiple comparisons.****p < 0.0001.

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