Table 2 Phenotypic NAI and CENI susceptibility of bovine HPAI A(H5N1) 2.3.4.4b viruses
Influenza A virus | NA/PA substitution | CENI baloxavir EC50 ± SD [nM] (fold change)a | NAI, IC50 ± SD [nM] (fold change)a | ||
|---|---|---|---|---|---|
Oseltamivir | Zanamivir | Peramivir | |||
bovine/TX/38161 | — | 0.52 ± 0.19 (2) | 3.08 ± 0.08 (2) | 0.29 ± 0.03 (1) | 0.08 ± 0.00 (1) |
bovine/TX/97794 | — | 0.19 ± 0.07 (1) | 0.70 ± 0.07 (1) | 0.14 ± 0.01 (1) | 0.07 ± 0.00 (1) |
bovine/TX/43134 | — | 0.34 ± 0.08 (1) | 0.71 ± 0.07 (1) | 0.14 ± 0.01 (1) | 0.07 ± 0.01 (1) |
bovine/TX/42041 | — | 0.65 ± 0.03 (2) | 0.91 ± 0.05 (1) | 0.15 ± 0.00 (1) | 0.07 ± 0.01 (1) |
bovine/OH/302 | — | 0.41 ± 0.16 (1) | 0.80 ± 0.13 (1) | 0.16 ± 0.02 (1) | 0.08 ± 0.01 (1) |
bovine/OH/358 | — | 0.52 ± 0.14 (2) | 0.84 ± 0.10 (1) | 0.20 ± 0.04 (1) | 0.09 ± 0.00 (1) |
bovine/OH/439 | — | 0.45 ± 0.05 (2) | 0.78 ± 0.05 (1) | 0.15 ± 0.00 (1) | 0.15 ± 0.06 (1) |
bovine/OH/541 | — | 0.17 ± 0.01 (1) | 0.98 ± 0.12 (1) | 0.19 ± 0.01 (1) | 0.09 ± 0.00 (1) |
Median (n = 8) | — | 0.43 | 0.82 | 0.15 | 0.08 |
A(H1N1)pdm09 reference viruses | |||||
A/Denmark/524/2009 | — | 0.72 ± 0.31 | 0.32 ± 0.14 | 0.17 ± 0.02 | 0.09 ± 0.00 |
A/Denmark/528/2009 | NA-H275Y | 0.63 ± 0.20 (1) | 125.38 ± 16.31 (86) | 0.20 ± 0.05 (1) | 25.69 ± 3.82 (285) |
A/Illinois/08/2018 | — | 0.57 ± 0.15 | 0.23 ± 0.01 | 0.29 ± 0.00 | 0.08 ± 0.01 |
A/Illinois/08/2018 | PA-I38T | 31.18 ± 11.85 (55) | 0.25 ± 0.03 (1) | 0.31 ± 0.01 (1) | 0.11 ± 0.00 (1) |
