Fig. 10: Mitochondrial function, inflammatory responses, and cell survival are slightly disrupted in Atg7 cKO macrophages during NTM infection in vitro.

a, b BMDMs from Atg7 cWT and Atg7 cKO mice were infected with Mabc-R (MOI of 5) for 6 h. Cells were stained with mitotracker (red), LC3 (green), and LAMP1 (green). Representative confocal images (a). Mitotracker/LC3 and mitotracker/LAMP1 colocalization were assessed by calculating Pearson’s coefficient (b, n = 10; independent biological replicates). c, d BMDMs from Atg7 cWT and Atg7 cKO mice were infected with Mabc-S (MOI of 5) or Mabc-R (MOI of 5) for 6 h. Cells were stained with MitoSOX (red) and DAPI (for nuclei). Representative confocal images (c). Average mean fluorescence intensities (MFI) of MitoSOX (d, n = 12; independent biological replicates). e Atg7 cWT and Atg7 cKO BMDMs were infected with Mabc-R (MOI of 1) for 96 h. Annexin V/PI analysis was measured by flow cytometry. Statistical significance was determined by two-tailed unpaired Student’s t-test or unpaired Student’s t-test with Welch’s correction (b, d), Welch’s ANOVA (P < 0.0001), followed by Dunnett’s T3 multiple comparisons test (d), and a two-tailed Student’s t-test (e). Data shown (means ± SEM) represent the combined results of triplicates from three independent experiments (b, d, e). Images are representative of three independent experiments (a, c). Un uninfected. Source data are provided as a Source Data file.