Fig. 4: FGFR1-altered lines are enriched in similar neuronal signatures observed in patient tumors. | Nature Communications

Fig. 4: FGFR1-altered lines are enriched in similar neuronal signatures observed in patient tumors.

From: A diverse landscape of FGFR alterations and co-mutations suggests potential therapeutic strategies in pediatric low-grade gliomas

Fig. 4

a Heatmap of the top 25 genes differentially expressed (DE) in the FGFR1 vs BRAF-altered mNSC lines. Gene names highlighted in orange represent genes involved in neuronal development. b Horizontal bar plots depicting the top 5 significant Gene Ontology C5 (MsigDB) Terms enriched (ranked by significance) in FGFR1 (n = 4) or BRAF- (n = 2) altered mNSC lines. c Venn diagram depicting the overlap of the top 20 significantly enriched C5 gene sets in the FGFR1-altered patient tumors and FGFR1-altered mNSC lines (n = 5 gene sets intersected). Significance was calculated by performing a two-sided Fisher’s exact test (p = 2.6e-11). Red text in the overlap list highlights gene sets that were also enriched in the FGFR1 SV-altered ihNSC lines. d UMAP embedding of all FGFR1-altered mNSC lines, colored by line. N = 4 samples, 19,009 cells. e UMAP embeddings of the FGFR1-altered mNSCs colored by the GO Neurogenesis gene set. Legend depicts gene set score. N = 4 samples, 19,009 cells. Abbreviations for models= F1-N546K: FGFR1 N546K SNV, F1-N546K + PTPN11: FGFR1 N546K SNV + PTPN11 E69K SNV, F1-ITD: FGFR1-ITD, F1::TACC1: FGFR1::TACC1, V600E: BRAF V600E SNV, K::B: KIAA1549::BRAF. Source data provided in Supplementary Data S4 and the Source Data File.

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