Fig. 4: SLAMF1 expression in infected macrophages depends on adaptive immunity and autophagy.

A–E WT, Rag1^−/−, and Tcra^−/− mice were infected with ~100 CFU mCherry Mtb for 4 weeks. A–C SLAMF1 positivity on infected and uninfected monocytes and non-AMs is shown. P value from two-way ANOVA Mixed-effects model with Tukey’s multiple comparisons test. D, E SLAMF1 positivity of infected and uninfected monocytes and CD11c^- non-AMs separated by MHCII status. P value from two-way ANOVA with Dunnett test. (A-E) n = 9 mice in each group. Data from 3 independent experiments. F–H Tcra^−/− mice were infected with ~100 CFU mCherry Mtb, and naïve T cells from WT mice were transferred 6 dpi. Lungs were harvested 5 wpi; F–H SLAMF1 positivity in Mtb-infected and uninfected monocytes and non-AMs. (I, J) SLAMF1 positivity of infected and uninfected monocytes and CD11c^- non-AMs separated by MHCII status. F–J Tcra^−/− n = 9 mice; Tcra^−/− + WT/T cells n = 10 mice. Data is from 2 independent experiments. P values from two-way ANOVA. K, L Atg5^fl/fl, Atg7^fl/fl, Atg14^fl/fl Cre + , and Cre- littermate controls infected with ~1000 CFU GFP-Mtb for 17 days. K SLAMF1 positivity in Mtb-infected (GFP^pos) and uninfected (GFP^neg) monocytes and macrophages from Atg Cre^- mice. n = 15 mice from 4 independent experiments. P value from two-way ANOVA with Sidak multiple comparisons test. Each data point represents one mouse; lines connect data from the same mouse. L SLAMF1 positivity in Mtb-infected and uninfected monocytes and macrophages from Cre- and Cre+ mice, as indicated. Cre- mice (Atg5^fl/fl, Atg7^fl/fl, Atg14^fl/fl) were combined for the analysis. Cre- n = 15 mice; ATG5 cKO n = 10 mice; ATG7 cKO n = 9 mice; ATG14 cKO n = 8 mice from 2–3 independent experiments. P value from two-way ANOVA with Dunnett multiple comparisons test. (A–J, L) Each data point represents one mouse. A–J, L Data presented as mean values ± SEM. Source data are provided as a Source Data file.