Fig. 5: SLAMF1 confers protection against Mtb.
From: Macrophage-T cell interactions promote SLAMF1 expression for enhanced TB defense
A, B WT and Slamf1−/− mice were infected with ~150 CFU H37Rv Mtb. Representative lung histopathology at 2, 4, and 8 weeks post-infection (wpi). Slamf1−/− mice had prominent neutrophilic inflammation at 4 and 8 wpi. Scale bars = 200 μm. n = 5 mice per group. Data from one experiment. B Representative H&E and acid-fast staining at 4 and 8 wpi shows increased inflammation in Slamf1−/− as compared to WT mice. Scale bars = 20 μm (H&E); 10 μm (AFB). C–H WT and Slamf1−/− mice were infected with ~1000 CFU H37Rv Mtb. C Lung Mtb burden was assessed 4 wpi. n = 22 mice. P value from two-tailed nonparametric Mann-Whitney t test. Data presented as mean values ± SEM. Different colors indicate independent experiments. D Survival was monitored (n = 15 mice per group). P value from Kaplan-Meier simple survival analysis. E–G Lungs were harvested and analyzed by flow cytometry. E Total number of different myeloid cells in the lung. F Number of Mtb-infected myeloid subsets. G Number of lymphoid cells. H Total number and percentages of antigen-specific CD4+ and CD8+ T cells. I Cytokine levels in the lung homogenates. E–G, I Each data point represents one mouse. Data consists of 12 mice from 3 independent experiments. E **** p = 2 × 10−15, F **** p = 1.3 × 10−7, and G **** p = 7.5 × 10−13 comparing WT to Slamf1−/− using Kruskal-Wallis. E–G, I Pairwise comparisons used the multiple Mann-Whitney tests. H n = 10–18 mice. P value from Mann-Whitney t test. E–I Data presented as mean values ± SEM. Source data are provided as a Source Data file.
