Fig. 4: Validation of cardiomyocyte CD36 expression. | Nature Communications

Fig. 4: Validation of cardiomyocyte CD36 expression.

From: Inhibiting CD36 palmitoylation improves cardiac function post-infarction by regulating lipid metabolic homeostasis and autophagy

Fig. 4

a Western-blot analysis of CD36 in different fractions of myocardium. n = 9 for total CD36; n = 5 for plasma membrane CD36; n = 8 for subcellular CD36. b CD36 mRNA level in isolated adult cardiomyocytes from Sham and MI mice. n = 6. c Western-blot analysis of CD36 in different fractions of isolated adult cardiomyocytes from Sham and MI mice. n = 6 for total CD36 and plasma membrane CD36; n = 8 for subcellular CD36. d Immunostaining assay was used to analyze the colocalization of CD36 (green) and Na+/K+-ATPase (red) in isolated adult cardiomyocytes. Trace outline is used for line-scan analysis of relative fluorescence intensities of CD36 with Na+/K+-ATPase signals. Scale bar = 100 µm. Data are presented as means ± SEM. Statistical significance was assessed by a two-tailed unpaired Student t test (a–c). Source data are provided as a Source Data file.

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