Fig. 7: Palmitoylation is essential for the plasma membrane localization of CD36 and has an impact on mitochondrial function and FA-uptaking in cardiomyocytes.

a Total CD36 protein expression in cardiomyocyte (NMVCs) was detected by FACS. n = 6 per group. b The plasma membrane and cytoplasm fraction were isolated from cardiomyocytes (NMVCs), and the relative distribution of CD36 protein in different fractions was analyzed by western blot. n = 6 for surface CD36; n = 10 for subcellular CD36. c, d (c) The dual-parameter contour plot correlating FL-C16 uptaking against CD36 expression, as well as a histogram showing the Mean Fluorescence Intensity (MFI) for each group. n = 7. d Cysteine mutation in the palmitoylation site of CD36 reduced FA transport. The blue points plotted along the x-axis indicate significant differences in FA uptaking values between WT-CD36 and AA-SS-CD36 cells with similar CD36 expression levels (determined by Student’s t tests reaching the 99.9% confidence value). e, f Ceramide (e) and DAG (f) levels in cardiomyocytes (NMVCs) transfected with WT-CD36 and AA-SS-CD36 after 24 h of hypoxia induction. n = 5 for ceramide; n = 7 for DAG. g The mitochondrial oxygen consumption rate (OCR) in cardiomyocytes (NMVCs) transfected with WT-CD36 and AA-SS-CD36 after 24 h of hypoxia induction. n = 5 per group. h Mean fluorescence intensity of MitoSOX in cardiomyocytes (NMVCs) transfected with WT-CD36 and AA-SS-CD36 after 24 h of hypoxia induction. n = 6 per group. Data are presented as means ± SEM. Statistical significance was assessed by One-way ANOVA, followed by Tukey post hoc multicomparisons test (a and e–h), two-tailed Mann-Whitney U test (b [surface CD36], c) and two-tailed unpaired t test (b [subcellular CD36]). Source data are provided as a Source Data file.