Fig. 1: Strategies and mechanisms for reduction of unsaturated compounds. | Nature Communications

Fig. 1: Strategies and mechanisms for reduction of unsaturated compounds.

From: Cofactor-independent photo-enzymatic reductions with water mediated by reductive graphene quantum dots

Fig. 1

a Strategies for the bioreduction of unsaturated compounds and the catalytic mechanism of AKR-catalyzed 3,5-BTPE reduction. During the enzymatic catalysis, ternary AKR-NADPH-substrate complexes form and the NADPH binding induces protonation of Tyr to form the catalytically active Tyr-OH₂⁺ species. Then, the common coenzyme binding domain of AKR permits pro-R-hydride from NADPH transfer to the carbonyl through protonation of the carbonyl by the TyrOH2+. Finally, the oxidized coenzymes NADP+ and chiral product leave. b NIR-driven photo-enzyme-coupled catalysis for 3,5-BTAP reduction. c This work, strategies for the light-driven photo-enzymatic reduction of unsaturated compounds by rGQDs/reductase photo-biocatalyst system.

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