Fig. 5: Standard reaction conditions for 3,5-BTAP reduction: a 5 mL reaction system consisting of 3,5-BTAP (32.3 mg, 0.125 mmol), 10 mg of rGQDs/AKR, isopropanol (3 μL), and 5 mL of PBS buffer (pH = 7.0, 100 mM) was performed at a quartz reactor, stirring at 20 °C under light irradiation for 18 h. | Nature Communications

Fig. 5: Standard reaction conditions for 3,5-BTAP reduction: a 5 mL reaction system consisting of 3,5-BTAP (32.3 mg, 0.125 mmol), 10 mg of rGQDs/AKR, isopropanol (3 μL), and 5 mL of PBS buffer (pH = 7.0, 100 mM) was performed at a quartz reactor, stirring at 20 °C under light irradiation for 18 h.

From: Cofactor-independent photo-enzymatic reductions with water mediated by reductive graphene quantum dots

Fig. 5

a HPLC spectrum of the product. (R)−3,5-BTPE yields of (b) using different catalyst systems including AKR, rGQDs, rGQDs/AKR, rGQDs/AKR + NADP+, and rGQDs/AKR + NADP+ + M (M denotes [Cp*Rh(bpy)(H2O)]2+) with IR light ( > 800 nm, 50 mW·cm-2) irradiation, (c) using different light sources, (d) using different wavelength LED lamps as light sources, (e) using infrared light (λ > 800 nm) as light sources with different light intensities, (f) using different rGQDs loading amounts at IR light irradiation. g Photo-enzymatic reduction of 1-methyl-3,4-dihydroisoquinolin and cinnamaldehyde with imine reductase AoIRED and ene reductase OYE1 as the corresponding biocatalyst, respectively. h Reduction of 3-chloro-1-phenylpropan-1-one using different catalyst systems, including rGQDs/carbonyl reductase (NaCBR), TiO2/NaCBR and TiO2-rGQDs/NaCBR, under corresponding photo-enzymatic reaction conditions. (i) Reuse of TiO2-rGQDs/NaCBR in cyclic catalysis. The error bars represent the standard deviations of three parallel measurements.

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