Fig. 7: PPH-PEI/GalNAc@siRubicon targets hepatocytes in an oxidative stress-responsive manner. | Nature Communications

Fig. 7: PPH-PEI/GalNAc@siRubicon targets hepatocytes in an oxidative stress-responsive manner.

From: Dual-targeted siRubicon delivery strategy triggers hepatocellular lipophagy for mitigating liver steatosis

Fig. 7

a Western blot analysis of Rubicon expression in livers of normal diet and HFD/MCD diet mice and b semi-quantitative analysis (n = 3). c changes in MDA expression in livers from normal and HFD/MCD diets (n = 6). d RT-qPCR analysis of pro-inflammatory cytokines IL-1β, (e) TNF-α, (f) IL-6, and (g) iNOS gene expression in livers from normal and HFD/MCD diet (n = 7). h relative fluorescence expression of PPH-PEI/GalNAc@Cy5-siRubicon in different organs at 2 and 12 h post-injection in mice fed with normal or HFD/MCD diet. i relative fluorescence expression of PPH-PEI/GalNAc@Cy5-siRubicon in different organs at 2 and 12 h post-injection in MCD-fed mice with or without antioxidant treatment. j Western blot analysis of the efficiency of siRubicon on hepatocytes in HFD/MCD-fed mice and k the relative expression quantification (n = 4). ** p < 0.01, and *** p < 0.001, insignificance was not represented. Error bars indicate means ± SEM in (b–g, and k). Data were analyzed by two-tailed Student’s t-test. Source data are provided as a Source Data file.

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