Fig. 5: The 1200–1400 nucleotides of CYP17A1 mRNA are responsible for binding to ZIKV-NS2A. | Nature Communications

Fig. 5: The 1200–1400 nucleotides of CYP17A1 mRNA are responsible for binding to ZIKV-NS2A.

From: Zika virus disrupts steroidogenesis and impairs spermatogenesis by stalling the translation of CYP17A1 mRNA

Fig. 5

a PAR-CLIP workflow71. See text for detail. b PAR-CLIP result summary. Uridines in the CYP17A1 mRNA that may participate in NS2A-binding were indicated. c, d A competition assay was performed by adding various unbiotinylated CYP17A1 mRNA fragments to the mixture of GST-NS2A and CYP17A1 mRNA-biotin. The remaining amounts of GST-NS2A pulled down by CYP17A1 mRNA-biotin were detected by Western blotting. e Schematic representation of distinct truncated hCYP17A1 mRNA. The nucleotides positions of each truncated hCYP17A1 mRNA are indicated. f HEK293T cells were co-transfected with plasmids encoding distinct truncated hCYP17A1-HA and NS2A-Flag. After 36 h post-transfection, the expression of hCYP17A1-HA and NS2A were detected by Western blotting. The images in c, d, f are representative of three independent experiments.

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