Fig. 1: GSDME deficiency aggravates cisplatin nephrotoxicity.

a Representative immunofluorescence images showing co-staining of GSDME (red) with LTL (green) in renal sections from mice on days 0-3 after cisplatin treatment. b Quantification of GSDME immunofluorescence intensity in panel (a) (n = 12 biological replicates). c RT-qPCR analysis of Gsdme in renal tissues from mice on days 0-3 after cisplatin treatment (n = 12 biological replicates). d, e Western blot analysis (d) and densitometric quantification (e) of GSDME protein levels in renal tissues from mice on days 0-3 after cisplatin treatment (n = 12 biological replicates). f Serum creatinine levels in WT and Gsdme-KO mice on days 0-3 after cisplatin treatment (n = 12 biological replicates). g,h Representative images of H&E staining (g) and corresponding renal tubular injury scoring (h) of renal sections from WT and Gsdme-KO mice on days 0-3 after cisplatin treatment (n = 12 biological replicates). i,j Western blot analysis (i) and densitometric quantification (j) of NGAL and GSDME protein levels in renal tissues from WT and Gsdme-KO mice on day 3 after cisplatin treatment (n = 12 biological replicates). k RT-qPCR analysis of Tnf, Ccl2, Il6, and Tlr4 mRNA expression in renal tissues from WT and Gsdme-KO mice on day 3 after cisplatin treatment (n = 12 biological replicates). l–n Representative immunohistochemistry staining (l) of F4/80 and MPO in renal sections from WT and Gsdme-KO mice on day 3 after cisplatin treatment, with quantitative analysis of F4/80 (m) and MPO (n) positive cells (n = 12 biological replicates). Data were expressed as means ± SEM. ^##P < 0.01, ^###P < 0.001 versus WT mice treated with saline. ^&P < 0.05 versus cisplatin-treated WT mice (day 2). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 versus cisplatin-treated WT mice (day 3). P-values were determined by one-way ANOVA (with Least-Significant Difference [LSD] or Games-Howell post hoc tests) in panels (b, c, e, f, j, k, m, n). P values were determined by a two-tailed nonparametric test (with Wilcoxon test) in panel (h). All scale bars, 100 μm. Source data are provided as a Source Data file.