Fig. 2: Cell populations and cellular attributes of WT and Gsdme-KO mouse kidneys revealed by scRNA-seq analysis. | Nature Communications

Fig. 2: Cell populations and cellular attributes of WT and Gsdme-KO mouse kidneys revealed by scRNA-seq analysis.

From: Renal tubular GSDME protects cisplatin nephrotoxicity by impeding OGT-STAT3-S100A7A axis in male mice

Fig. 2

a UMAP visualization of single-cell transcriptomes from kidneys of Gsdme-KO mice and their WT littermates. Colors represent distinct cell clusters; each dot corresponds to a single cell. b UMAP plot displaying the subclusters of PTCs. c Bubble plot showing the gene expression profiles of Gsdme across different renal cell populations. PTCs Proximal Tubular Cells, MPC Mono-nuclear Phagocyte, T cells T lymphocytes, Neutro Neutrophil, B cells B lymphocytes, CD Collecting Duct, LOH Loop of Henle, ECs Endothelial Cells, DCT Distal Convoluted tubule, NK cells Natural Killer cells, Myofib Myofibroblasts, PECs/Podo Parietal Epithelial Cells/Podocyte. d Bubble plot showing the gene expression profiles of Gsdme across different WT PTCs subpopulations. e Proportions of different PTCs subpopulations in different groups. f Pseudotime analysis illustrating the differentiation trajectory of PTCs. g Visualization of PTCs differentiation trajectories stratified by the indicated experimental condition. h Heatmap showing differentially expressed genes (DEGs, rows) along pseudotime (columns), hierarchically clustered into four distinct expression patterns, each corresponding to a specific trajectory phase. Right panel: GO-BP terms associated with each gene expression pattern.

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