Fig. 6: S100A7A exacerbates cisplatin nephrotoxicity by recruiting inflammatory cells via its receptor RAGE. | Nature Communications

Fig. 6: S100A7A exacerbates cisplatin nephrotoxicity by recruiting inflammatory cells via its receptor RAGE.

From: Renal tubular GSDME protects cisplatin nephrotoxicity by impeding OGT-STAT3-S100A7A axis in male mice

Fig. 6

a, b Western blot analysis (a) and densitometric quantification (b) of RAGE protein levels from cisplatin-treated WT and Gsdme-KO mice (n = 12 biological replicates). c RT-qPCR analysis of Ager, Tnf, Il6, Cd86, and Mrc1 mRNA expression in BMDMs treated with or without recombinant S100A7A protein (n = 4 biological replicates). d Representative transwell images showing migration of BMDMs treated with the indicated concentrations of recombinant S100A7A protein. e Quantitative analysis of BMDMs migration from experiments in panel (d) (n = 4 biological replicates). f Representative transwell images showing BMDM migration in response to culture supernatants collected from PTCs of different genotypes with various treatments (cisplatin, S100a7a siRNA, and FPS-ZM1). g Quantitative analysis of BMDMs migration from experiments in panel (f) (n = 4 biological replicates). h Serum creatinine levels in cisplatin-treated WT and Gsdme-KO mice, with or without FPS-ZM1 pretreatment (n = 8 biological replicates). i, j Representative images of H&E staining (i) and histopathology scoring (j) of renal sections in cisplatin-treated WT and Gsdme-KO mice, with or without FPS-ZM1 pretreatment (n = 8 biological replicates). k RT-qPCR analysis of Tnf and Il6 mRNA expression in renal tissues from cisplatin-treated WT and Gsdme-KO mice, with or without FPS-ZM1 pretreatment (n = 8 biological replicates). l–o Representative immunohistochemistry staining of F4/80 (l) and MPO (m) in renal sections from cisplatin-treated WT and Gsdme-KO mice, with or without FPS-ZM1 pretreatment. Quantitative analysis of F4/80 (n) and MPO positive cells (o) (n = 8 biological replicates). Data were expressed as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 versus cisplatin-treated WT mice. #P < 0.05, ##P < 0.01, ###P < 0.001 versus cisplatin-treated Gsdme-KO mice pretreated with FPS-ZM1. & &P < 0.01, & & &P < 0.001 versus BMDMs ctrl group. P-values were determined by Student’s two-tailed t-test in (c) and one-way ANOVA (with LSD or Games-Howell post hoc tests) in (b, e, g, h, k, n, o). P values were determined by a two-tailed nonparametric test (Wilcoxon test) in (j). All scale bars, 100 μm. Source data are provided as a Source Data file.

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