Fig. 4: Impairing the Erg11-Ncp1 interaction triggers ER stress.
a Go pathway enrichment analysis using RNA-seq to compare the transcriptome data of the ERG11/erg11Δ and erg11m/erg11Δ mutants after 16 h at 30 °C. The Q-value, which is the P-value adjusted for multiple hypothesis testing, ranges from 0 to 1. A lower Q-value indicates a higher level of significance in terms of enrichment. The Rich Ratio is calculated as the ratio of differentially expressed genes within a pathway term to all annotated genes within that pathway term. b Cellular levels of ROS were measured in the absence of drug treatment and following treatment with 8 μg/mL menadione for 4 h. c Quantification of cellular misfolded proteins was conducted by treating mutants ERG11/erg11Δ and erg11m/erg11Δ without or with 4 μg/mL FLC. d The mRNA expression of the HAC1 and IRE1 genes in the absence or presence of 4 μg/mL FLC for 4 h was quantified. The mutant ERG11/erg11Δ was treated with 5 mM DTT for 2 h as the positive control. The ACT1 gene served as the control. Three biological replicates were carried out. Data were presented as mean ± SD (n = 3). Statistical analysis was performed using an unpaired two-tailed t test (b, c, d). Source data are provided as a Source Data file.
