Fig. 6: The impaired Erg11-Ncp1 interaction leads to mitochondrial dysfunction. | Nature Communications

Fig. 6: The impaired Erg11-Ncp1 interaction leads to mitochondrial dysfunction.

From: Impairing the interaction between Erg11 and cytochrome P450 reductase Ncp1 enhances azoles’ antifungal activities

Fig. 6

a Rhodamine 123 staining was utilized to observe alterations in mitochondrial membrane potential. A positive control group that the ERG11/erg11Δ mutant treated with 5 mM DTT for 2 h was included. b A quantitative analysis was conducted on mitochondrial ROS levels in the ERG11/erg11Δ and erg11m/erg11Δ mutants. A positive control group was included, consisting of the ERG11/e rg11Δ mutant incubated with 5 mM DTT for 2 h. c The fluorescence intensity of misfolded proteins within mitochondria was assessed using flow cytometry. A positive control group that the ERG11/erg11Δ mutant was treated with DTT was included. d Density plots were used to visualize the fluorescence patterns of the ERG11/erg11Δ and erg11m/erg11Δ mutants for 8 h. Data were obtained from three biological repeats, and representative images are shown. e Quantification of Annexin V/PI double staining assay. Data are mean ± SD of n = 3 biological replicates (a, c, e). Two-tailed unpaired t-tests were used (a, c, e). Source data are provided as a Source Data file.

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