Fig. 2: DSS1 suppresses autophagic flux in ccRCC cells. | Nature Communications

Fig. 2: DSS1 suppresses autophagic flux in ccRCC cells.

From: DSS1 inhibits autophagy to activate epithelial-mesenchymal transition in a pro-metastatic niche of renal cell carcinoma

Fig. 2

a Schematic diagram illustrating KEGG autophagy pathway negatively associated with DSS1 expression (DESeq2 log2CPM). b Transmission electron microscopy (TEM, left) images and quantification (right) of autophagic vacuoles (arrows) in ACHN and Caki-1 cells transfected with siDSS1 or siNC (n = 3 independent experiments; scale bars: 8000×, 2 μm, 20000×, 0.5 μm; boxes: zoom-in regions; error bar: mean ± SD; two-tailed Welch’s t-test, Benjamini-Hochberg [BH] adjustment). c Confocal microscopy images of LC3 puncta (magenta) and LAMP1 (turquoise) co-localization in cells treated with siNC, siDSS1, with or without chloroquine (CQ: 10 μM, control: 0.02% DMSO, 24 h before harvest; n = 3 independent experiments; scale bar: 10 μm; error bar: mean ± SD; two-tailed Welch’s t-test, BH adjustment). COSMIC, Catalogue of Somatic Mutations in Cancer. Statistics are provided in the source data. Source data are provided as a Source Data file.

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