Fig. 3: dMterf4 RNAi downregulates LManVI transcription via Med8/Tfb4 to E(z)/pho axis.

a Knockdown of Med8 and Tfb4 increased LManVI transcription in dMterf4 knockdown flies. b–e Knockdown of E(z) and pho partially rescued the abnormal wing postures (b), climbing impairment (c), decreased ATP levels (d), and shortened lifespan (e) caused by knockdown of dMterf4. f Knockdown of Med8 and Tfb4 decreased the transcription levels of E(z) and pho in dMterf4 knockdown flies. g Knockdown of E(z) or pho increased LManVI mRNA level in dMterf4 knockdown flies. h knockdown of E(z) and pho could reverse Fg-Med8 and Fg-Tfb4 overexpression-mediated LManVI downregulation in dMterf4 RNAi background. i Fg-E(z) interacted with HA-pho in S2 cells (Actin: loading control, representative pictures were shown). j In dMterf4 RNAi background, simultaneous overexpression of Fg-E(z) and knockdown of pho showed similar phenotype to pho RNAi alone. k, l Knockdown of Esc and Caf1–55 partially rescued the abnormal wing postures (k) and decreased ATP levels (l) caused by knockdown of dMterf4. m In dMterf4 RNAi background, the downregulation of LManVI expression caused by overexpression of Fg-E(z) could be reversed by Esc RNAi and Caf1–55 RNAi. The error bars are mean ± SD. All data were performed with three biological replicates (n = 3). For sample sizes: n = 10 fly thoraxes (a, f, g, h, j, and m), 100 flies (b, c, and k), or 6 fly thoraxes (d and l) were used in each replicate. For lifespan, sample sizes were shown in the figure (e). For the statistics, one-way ANOVA with Tukey’s multiple comparisons test was used. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns means no significant difference. Data points indicate biological replicates. Source data were provided as a Source Data file.