Fig. 4: Knockdown of LManVI impairs mitochondrial function.

a Knockdown of LManVI resulted in abnormal mitochondrial morphology (representative pictures were shown). b Quantification of mitochondrial sizes of (a). n = 185 and 186 (from left to right) individual mitochondria were analyzed with ImageJ. c The top panel showed fly muscles mitochondrial morphologies of control and knockdown of LManVI flies observed by TEM. The lower panel showed the higher magnification of images in the red box of corresponding top panel (representative pictures were shown). d Quantification of mitochondrial sizes of (c). n = 185 and 186 (from left to right) individual mitochondria were analyzed with ImageJ. e, f The mRNA levels of nuclear-encoded (e) and mitochondria-encoded (f) mitochondrial genes were decreased after knockdown of LManVI. g, h Knockdown of LManVI with Mef2-gal4 also downregulated the mRNA levels of nuclear-encoded (g) and mitochondria-encoded (h) mitochondrial genes. i The mRNA levels of mitochondrial transcription-related factors mtTFB2, mtTFA, and PGC-1 were decreased after knockdown of LManVI. j Knockdown of LManVI decreased fly ATP levels. k Knockdown of LManVI weakened fly climbing ability. n = 124 (w1118) and 118 (LManVI RNAi) flies were counted. All data were performed with three biological replicates (n = 3). For sample sizes: n = 6 fly thoraxes (a, j), 3 fly thoraxes (c), 10 flies (e, f, and i), or 10 fly thoraxes (g and h) were used in each replicate. Data points indicate individual mitochondria (b, d) or biological replicates (e–j). The error bars are mean ± SD. For the statistics, two-tailed unpaired t-test was used. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns means no significant difference. Scale bars: a, 10 μm; c, 0.5 μm and 0.2 μm. Source data were provided as a Source Data file.