Fig. 5: In-depth snRNA-seq. analysis predicts CM4 as a progenitor population of CMs. | Nature Communications

Fig. 5: In-depth snRNA-seq. analysis predicts CM4 as a progenitor population of CMs.

From: Single-nuclei multiomics analysis identifies abnormal cardiomyocytes in a murine model of cardiac development

Fig. 5

A RNA velocity analysis of CM subclusters. B Latent Time of RNA velocity. Purple is time 0, yellow is time 1. C Gene expression dynamics of marker genes in each subcluster ordered along latent time inferred by RNA velocity analysis. Analysis shows that Tbx5 is associated with an earlier latent time and CM4 cluster. D Top: Plot of Tbx5 and Mef2c expression across pseudotime for each subcluster of CM. Both factors show expression in CM4 cells and at earlier pseudotime. Bottom: Violin plot of Tbx5 and Mef2c across CM subclusters. Factors are enriched in the CM4 cluster (boxed regions). E Left: Plot of CM4 markers Nppa and Sox5 expression across pseudotime from each subcluster of CM. Right: Violin plot of Nppa and Sox5 in CM1 and CM4 clusters. CM4 markers are enriched in PMIS-miR-200 samples. F Immunofluorescent stain of apical left ventricle sections, at E14.5, for Sox5 (Green) and Nppa (Red). PMIS-C and PMIS-AC hearts show clear enrichment of cell expression of both Nppa and Sox5 (yellow arrows). Four independent experiments were performed with each showing similar results. Scale bar = 25μm.

Back to article page