Fig. 7: miR-200c regulates the TF Irx1 in CM4 cells. | Nature Communications

Fig. 7: miR-200c regulates the TF Irx1 in CM4 cells.

From: Single-nuclei multiomics analysis identifies abnormal cardiomyocytes in a murine model of cardiac development

Fig. 7

A Functional testing of the miR-200c regulation site in the Irx1 3’ UTR. N = 4. B qPCR for Irx1 expression in PMIS-miR-200 E14.5 hearts and PMIS transduced AC16 cells. RNA was isolated from whole hearts of litter mates used for snMulti-Omics experiment. N = 3. C Immunofluorescent stain of apical left ventricle sections, at E14.5, for Irx1 (Green) and cTnT (Red). PMIS-C and PMIS-AC hearts show clear enrichment of Irx1 expression (yellow arrowhead). Scale bar = 25 μm. D Visualization of ATAC-Seq reads of open chromatin around the Irx1 locus in the CM4 cluster from each PMIS sample. The Irx1 locus appears more open in PMIS-C and PMIS-AC samples compared to WT. E Gene expression dynamics of marker genes in each subcluster ordered along latent time inferred by RNA velocity analysis. Analysis shows that Tbx5 is associated with an earlier latent time in the CM4 cluster. F Immunofluorescent stain of apical left ventricle sections, at E14.5, for Irx1 (white), Tbx5, and Nppa (red) and cTnT (green). PMIS-C and PMIS-AC hearts show co-localization of Irx1 and Tbx5 and Irx1 and Nppa in CMs (yellow arrowhead). Scale bar = 25 μm. The statistic test performed was one-way ANOVAwith multiple comparisons. Data are presented as mean values ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.

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