Fig. 6: Allosteric sites at the mAChRs.
From: Cryo-EM reveals an extrahelical allosteric binding site at the M5 mAChR
a Model of the ACh-VU6007678 bound M5 mAChR showing the allosteric binding site discovered for VU6007678 (orange spheres) and the ECV allosteric site occupied by LY2119620 (salmon spheres). ACh is shown as green spheres in the orthosteric binding site. b Allosteric modulator structures overlaid on the VU allosteric site, with ligands shown as ball-and-stick models. D1:LY3154207:cholesterol in dark slate grey and slate grey, respectively (PDB:7X2F). β2AR:Cmpd-6FA in light sea green (PDB:6N48). FFAR1:AP8 in violet (PDB:5TZY). GPBAR1:INT-177 in purple (PDB:7CFN). CASR:NDT9513727 in salmon (PDB:6C1Q). C5aR:avacopan in green (PDB:6C1R). c Cryo-EM density (contour level 0.47) for lipid molecules observed at the bottom of TM2,3,4.
