Fig. 3: Complement receptor-related uEV (CMR-EV) subcluster exhibit diagnostic potential for sepsis-associated AKI.
A UMAP plot distribution of the 5 uEV subclusters with the most significant proportional differences (endothelial cell derived EV: EC-EV, Tubular epithelial-derived EV: TEC-EV, Complement receptor related EV: CMR-EV, Lysosome related EV: LYS-EV, immune response related EVs: IMR-EV); B UMAP plot distribution of these 5 subclusters between AKI and non-AKI groups; C Key characteristic proteins of the 5 uEV subclusters; D Proportional distribution patterns of the 5 uEV subclusters across AKI stage (stage 1 vs. stages 2-3) were resolved through sample-specific proportional weighting normalization; E Proportional distribution of the 5 uEV subclusters in transient and persistent AKI; F Radar chart highlighting CMR-uEV as the most significantly different cluster between SA-AKI and non-AKI groups (Mann–Whitney U two-sided test, SA-AKI, n = 8, Non-AKI, n = 8); G, H Second polynomial distribution revealed significant trends (ptrend = 0.047) in the proportional distribution of CMR-uEV in SA-AKI patients with different duration (G) (Non-AKI, n = 8; Transient-AKI, n = 3, Persistent-AKI, n = 5) and stages (H) (Non-AKI, n = 8; AKI-stage1, n = 2, AKI-stage2-3, n = 6). Data are presented as box plots showing the median (middle line), the 25th and 75th percentiles (box limits), the minimum and maximum values (whiskers), and outliers (individual points) Source data are provided as a Source Data file.
