Fig. 1: Experimental design.

a Housing conditions for each group. b Experimental schedule. Mice were placed in their environment at 28 d post-weaning (P28) for 7 weeks. Histology was performed immediately after the fMRI experiment. c Presence or absence of environmental conditions for each group. d Group comparisons and hypothesized effects between each housing condition. e Body weight measurements indicated a significantly greater increase in SS (n = 20; blue) than in SG (n = 20; red), and EG (n = 20; green) during 7-week change housing conditions (p < 0.05). Data are presented as the mean ± SEM (two-way ANOVA corrected with Tukey’s test; *p < 0.05, **p < 0. 01, ***p < 0. 001, ****p < 0. 0001).