Fig. 4: PIEZO1 is required for trophoblast fusion in vitro.
From: PIEZO1 drives trophoblast fusion and placental development
Representative images (A) and fusion index quantification (B) of untreated control (n = 6) and 5 μM GsMTx4 treated (n = 6) BeWo cells after 48-h forskolin treatment. Unpaired 2-sided Student t-test. ****: p = 0.000000576. Representative images (C) and fusion index quantification (D) of control (n = 8) and PIEZO1 siRNA knockdown (n = 8) BeWo cells after forskolin treatment for 48-h to induce fusion. Unpaired 2-sided Student t-test. ****: p = 0.00000384529. Representative images (E) and fusion index quantification (F) of BeWo cells that were overexpressed with empty vector (n = 8) and PIEZO1 (n = 8) after 48-h forskolin treatment. Unpaired 2-sided Student t-test. ****: p = 0.00002456791. Each dot represents fusion indexes averaged from six random fields of one coverslip. Data are presented as mean ± s.e.m. Source data are provided as a Source Data file.
