Fig. 1: Overview of the decompartmentalization-based cofactor engineering strategy. | Nature Communications

Fig. 1: Overview of the decompartmentalization-based cofactor engineering strategy.

From: Decompartmentalization of the yeast mitochondrial metabolism to improve chemical production in Issatchenkia orientalis

Fig. 1

A Compartmentalization relies on localizing a metabolic pathway from the cytosol to a subcellular organelle to exploit the organelle’s cofactor supply, while decompartmentalization focuses on localizing the enzymes contributing to the high level of cofactor from the organelle to the cytosol to increase the cytosolic cofactor provision. Dots indicate metabolites, while arrows indicate transport or enzymatic reactions. B A schematic diagram for enhanced SA production in I. orientalis through the decompartmentalization of mitochondrial metabolism. The genes in the rTCA pathway are marked in blue, while pyruvate dehydrogenase and the genes associated with the glyoxylate shunt are marked in red. OAA oxaloacetate, PYC pyruvate carboxylase, MDH malate dehydrogenase, FUMR fumarase, FRD fumarate reductase, PDH pyruvate dehydrogenase, CIT citrate synthase, ACO aconitase, MLS malate synthase, ICL isocitrate lyase.

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