Fig. 3: SsCm2 but not SsCm1 has chorismate mutase activity. | Nature Communications

Fig. 3: SsCm2 but not SsCm1 has chorismate mutase activity.

From: A fungal effector promotes infection via stabilizing a negative regulatory factor of chloroplast immunity

Fig. 3

a SsCm2 but not SsCm1 can complement the genetic defect of yeast aro7. SD-Leu synthetic medium (SD) without leucine, SD-Leu/Phe/Tyr SD without leucine, tyrosine, and phenylalanine. The pGADT7 was used as a negative control, and pGADT7-ScAro7 was used as a positive control. b SsCm2 but not SsCm1 has chorismate mutase activity in vitro. Recombinant SsCm1ΔSP-HA, SsCm2-HA, GST (negative control), and UmCmu1ΔSP-HA (positive control) were used to perform chorismate mutase activity assays. The absorbance of the phenylpyruvate was measured in alkaline solution at 320 nm at the indicated time points (min). c SsCm1 has no effect on the chorismate mutase activity of GmCms in vitro. The equal amount of recombinant GmCms-HA was incubated with GST or SsCm1ΔSP-HA, respectively, and the chorismate mutase activity assays were performed according to the methods in (b). For b, c, data represent the mean ± SD (n = 3 biological replicates), source data are provided as a Source Data file.

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