Fig. 8: Overexpression of SsCm1 or GmMORF2a alleviates photosynthetic inhibition under immunization.
Overexpression of GmMORF2a or SsCm1 in DD (DEX:AtMKK5DD) alleviates the decrease of Fv/Fm (a) and photosynthesis-related gene expression (b) after DEX treatment. 15 μM DEX was infiltrated into leaves and kept in light for 18 h to measure Fv/Fm and for 8 h to quantify gene transcript levels, EtOH was used as the solution control. c Overexpression of SsCm1 or GmMORF2a alleviates the decrease of Fv/Fm induced by Pst-AvrRpt2. Pst-AvrRpt2 (OD600 = 0.2) was infiltrated into leaves and Fv/Fm was measured in indicated periods of time. Overexpression of SsCm1 (d) or GmMORF2a (e) alleviates the decrease of photosynthesis-related genes induced by Pst-AvrRpt2. Pst-AvrRpt2 (OD600 = 0.2) or MgCl2 was infiltrated into leaves and gene transcript levels were quantified after 4 h. f Effects of deleting SsCm1 on the Fv/Fm of infected areas of leaves during S. sclerotiorum infection. The PDB or pure hyphae of Δoah or Δoah/ΔSsCm1-9 was inoculated onto leaves, and the area of inoculation site after designated time was collected for measuring the Fv/Fm. Overexpression of SsCm1 (g, i) or GmMORF2a (h, j) alleviates the decrease of Fv/Fm (g, h) and photosynthesis-related gene expression (i, j) induced by SCFE and UF-1. 50 µg/mL SCFE or PDB was infiltrated into leaves for 24 h to measure Fv/Fm and for 12 h to quantify gene transcript levels. Mycelia of UF-1 was inoculated onto the leaves for 6 h to measure Fv/Fm and for 4 h to quantify gene transcript levels. Data (mean ± SD) were analyzed by two-way (b, d–j) or one-way ANOVA (a) with multiple comparisons test, data points with different letters indicate significant differences of p < 0.05 (b, d, e, g–j), or with ns = no significance, **p < 0.01, and ****p < 0.0001 (compared to DD for a; comparison between Δoah and Δoah/ΔSsCm1-9 for f). N = 4 biological replicates (a, c, f–h) or 3 biological replicates (b, d, e, h–j). Source data are provided as a Source Data file.
