Fig. 3: Cardiomyocytes-specific MacroD1 knockout preserves mitochondrial bioenergetics in septic insult. | Nature Communications

Fig. 3: Cardiomyocytes-specific MacroD1 knockout preserves mitochondrial bioenergetics in septic insult.

From: Cardiomyocyte mitochondrial mono-ADP-ribosylation dictates cardiac tolerance to sepsis by configuring bioenergetic reserve in male mice

Fig. 3

A Heatmap showing the relative expression of genes involved in the indicated metabolism pathway in the hearts of Flox and cKO mice injected intraperitoneally with/without LPS (10 mg/kg) for 18 h. B, C Gene set enrichment analysis (GSEA) of genes involved in indicated metabolism and oxidative phosphorylation pathways in the hearts of Flox and cKO mice 18 hours after LPS injection. D mRNA expression of genes involved in indicated metabolism pathways in the hearts of mice (n = 4). E, F Representative dihydroethidium (DHE) fluorescent images and quantification of hearts from Flox and cKO mice with and without LPS administration for 18 h (n = 6). G Myocardial ATP contents from Flox and cKO mice 18 hours after saline or LPS injection (n = 6). H Representative transmission electron microscopy images of hearts from mice 18 h after LPS administration. I, J Quantification of cristae number/mitochondria (I; n = 85, 88, 97, and 84 mitochondria were analyzed in the Flox, cKO, Flox + LPS, and cKO + LPS groups, respectively.) and mitochondrial area (J; n = 352, 362, 389, and 384 mitochondria were analyzed in the Flox, cKO, Flox + LPS, and cKO + LPS groups, respectively) from (H). Each group consisted of 5 heart samples from different animals, with five randomly selected electron microscopy fields per sample for analysis of mitochondria. n represents the number of mitochondria. The box boundaries represent the 25th (Q1) and 75th percentiles (Q3) with a central line at the median (50th percentile). The box height spans the interquartile range (IQR = Q3–Q1). Whiskers extend to the lowest/highest data points within Q1–1.5 × IQR and Q3 + 1.5 × IQR bounds, respectively. Minima and maxima correspond to actual extreme values within the whisker-defined range. Data are shown as the mean ± SD for (D–G). Flox: MacroD1flox/flox controls; cKO: MacroD1flox/flox/αMHCMerCreMer mice. Data analysis was conducted utilizing one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

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