Fig. 4: Downregulation of MacroD1 counteracts mitochondrial dysfunction induced by LPS in cardiomyocytes. | Nature Communications

Fig. 4: Downregulation of MacroD1 counteracts mitochondrial dysfunction induced by LPS in cardiomyocytes.

From: Cardiomyocyte mitochondrial mono-ADP-ribosylation dictates cardiac tolerance to sepsis by configuring bioenergetic reserve in male mice

Fig. 4

Neonatal rat cardiomyocytes (NRCMs) transfected with MacroD1 SiRNAs (Si-M) or scrambled control (NC) for 36 h and then treated with and without LPS (1 μg/ml) for 18 h to examine mitochondrial function. A, B Representative fluorescence images and quantification of MitoSOX in NRCMs (n = 8). C Seahorse mitochondrial stress test was carried out in NRCMs (n = 8) (OCR: Oxygen consumption rate; Oligo: Oligomycin; Rot/AA: Rotenone/Antimycin). D–G Quantification of basal respiration (D), maximal respiration (E), ATP-linked respiration (F), and spare respiration capacity (G) from C (n = 8). H–K Measurements of oxygen consumption rate and corresponding indexes for seahorse fatty acid oxidation in NRCMs in the presence of bovine serum albumin (BSA) (n = 7) (ETO: Etomoxir, FAO inhibitor). L–O Oxygen consumption rate and corresponding indexes for seahorse fatty acid oxidation in NRCMs in the presence of BSA and palmitate (PAL) (n = 7). Data are shown as the mean ± SD. Data analysis was conducted utilizing one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

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