Fig. 1: Impact of the preNuc treatment for salivary metagenomics. | Nature Communications

Fig. 1: Impact of the preNuc treatment for salivary metagenomics.

From: Giant extrachromosomal element “Inocle” potentially expands the adaptive capacity of the human oral microbiome

Fig. 1

A Schematic workflow for high-molecular DNA extraction from human saliva samples. Blue arrows indicate processes of the preNuc. B Effect of preNuc treatment on the removal of human DNA from saliva samples. Each plot shows the percentage of human reads in NovaSeq reads obtained using preNuc-treated and untreated saliva samples. *p < 0.05, N.S., not significant based on two-sided paired Student’s t test. C Effect of preNuc treatment on the sequence depth of microbial-derived long reads. Bar plots show the total bases of PromethION reads without low-quality and human reads per flow cell acquired from preNuc-treated and untreated samples. D Effect of preNuc treatment on the sequence length of long reads. Bar plots show the N50 length of PromethION reads without low-quality and human reads per flow cell of the preNuc-treated and -untreated samples. E Effect of preNuc treatment on bacterial composition. Scatter plots show the correlation of species-level bacterial composition between preNuc-treated and untreated saliva samples.

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